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Prof. Dr. Ernst Stelzer

Institute of Cell Biology and Neuroscience

Goethe University Frankfurt,
Riedberg Campus, BMLS
Max-von-Laue-Str. 15
60438 Frankfurt am Main
Germany

Tel  +49 (0)69 798-42547
Fax +49 (0)69 798-42546

e-mail
website

Administrative Assistant

Michaela Koller

Goethe University FrankfurtCampus Riedberg, BMLSMax-von-Laue-Str. 1560438 Frankfurt am MainGermany

Tel +49 (0)69 798-42545
Fax +49 (0)69 798-42546

e-mail

Ernst Stelzer

Professor in Advanced Light Microscopy,
Institute of Cell Biology and Neuroscience


Curriculum Vitae

1983Diploma in Physics, Max-Planck-Institute for Biophysics,
Frankfurt am Main, Germany
1987PhD, University of Heidelberg, Germany
1987–1989Project leader EMBL Heidelberg, Germany
1989–2009Group Leader, EMBL Heidelberg, Germany
since 2009Professor in Advanced Light Microscopy,
Cluster of Excellence "Macromolecular Complexes",
Goethe University Frankfurt

Team

Ernst Stelzer, Group Leader
Elia Benito-Gutierrez, Postdoctoral Fellow
Fabian Härle, Software Engineer
Berit Langer, Technical Assistant
Hildegard Merkle, Administrative Assistant
Francesco Pampaloni, Staff Scientistmarko.reinhardt(at)physikalischebiologie.de
Patrick Theer, Physics Engineer
Daniel von Wangenheim, PhD Student
Nariman Ansari, Scientist and Project Leader ProMEBS

Publications

An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Rosquete, MR; von Wangenheim, D; Marhavý, P; Barbez, E; Stelzer, EH; Benková, E; Maizel, A; Kleine-Vehn, J; Curr Biol, 2013 May PubMed

Lateral root morphogenesis is dependent on the mechanical properties of the overlaying tissues. Lucas, M; Kenobi, K; von Wangenheim, D; Voβ, U; Swarup, K; De Smet, I; Van Damme, D; Lawrence, T; Péret, B; Moscardi, E; Barbeau, D; Godin, C; Salt, D; Guyomarc'h, S; Stelzer, EH; Maizel, A; Laplaze, L; Bennett, MJ; Proc Natl Acad Sci U S A, 2013 Mar PubMed

High-resolution deep imaging of live cellular spheroids with light-sheet-based fluorescence microscopy. Pampaloni, F; Ansari, N; Stelzer, EH; Cell Tissue Res, 2013 Apr PubMed

Quantitative 3D cell-based assay performed with cellular spheroids and fluorescence microscopy. Ansari, N; Müller, S; Stelzer, EH; Pampaloni, F; Methods Cell Biol, 2013 PubMed

Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope provides an improved signal to noise ratio. Greger, K; Neetz, MJ; Reynaud, EG; Stelzer, EH; Opt Express, 2011 Oct PubMed

Digital scanned laser light-sheet fluorescence microscopy (DSLM) of zebrafish and Drosophila embryonic development. Keller, PJ; Schmidt, AD; Wittbrodt, J; Stelzer, EH; Cold Spring Harb Protoc, 2011 Oct PubMed

Viscoelastic response of contractile filament bundles. Besser, A; Colombelli, J; Stelzer, EH; Schwarz, US; Phys Rev E Stat Nonlin Soft Matter Phys, 2011 May PubMed

High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy. Maizel, A; von Wangenheim, D; Federici, F; Haseloff, J; Stelzer, EH; Plant J, 2011 Oct PubMed

A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells. Tängemo, C; Ronchi, P; Colombelli, J; Haselmann, U; Simpson, JC; Antony, C; Stelzer, EH; Pepperkok, R; Reynaud, EG; J Cell Sci, 2011 Mar PubMed

ImFCS: a software for imaging FCS data analysis and visualization. Sankaran, J; Shi, X; Ho, LY; Stelzer, EH; Wohland, T; Opt Express, 2010 Dec PubMed

Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos. Redemann, S; Pecreaux, J; Goehring, NW; Khairy, K; Stelzer, EH; Hyman, AA; Howard, J; PLoS One, 2010 PubMed

Madin-Darby canine kidney cells are increased in aerobic glycolysis when cultured on flat and stiff collagen-coated surfaces rather than in physiological 3-D cultures. Pampaloni, F; Stelzer, EH; Leicht, S; Marcello, M; Proteomics, 2010 Oct PubMed

Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy. Keller, PJ; Schmidt, AD; Santella, A; Khairy, K; Bao, Z; Wittbrodt, J; Stelzer, EH; Nat Methods, 2010 Aug PubMed

Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Wohland, T; Shi, X; Sankaran, J; Stelzer, EH; Opt Express, 2010 May PubMed

Digital scanned laser light sheet fluorescence microscopy. Keller, PJ; Stelzer, EH; Cold Spring Harb Protoc, 2010 May PubMed

Nlcam modulates midline convergence during anterior neural plate morphogenesis. Brown, KE; Keller, PJ; Ramialison, M; Rembold, M; Stelzer, EH; Loosli, F; Wittbrodt, J; Dev Biol, 2010 Mar PubMed

A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation. Timinszky, G; Till, S; Hassa, PO; Hothorn, M; Kustatscher, G; Nijmeijer, B; Colombelli, J; Altmeyer, M; Stelzer, EH; Scheffzek, K; Hottiger, MO; Ladurner, AG; Nat Struct Mol Biol, 2009 Sep PubMed

Three-dimensional tissue models for drug discovery and toxicology. Pampaloni, F; Stelzer, EH; Masotti, A; Recent Pat Biotechnol, 2009 PubMed

Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage. Reynaud, EG; Krzic, U; Greger, K; Stelzer, EH; HFSP J, 2008 Oct PubMed

Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization. Colombelli, J; Besser, A; Kress, H; Reynaud, EG; Girard, P; Caussinus, E; Haselmann, U; Small, JV; Schwarz, US; Stelzer, EH; J Cell Sci, 2009 May PubMed

Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy. Keller, PJ; Stelzer, EH; Curr Opin Neurobiol, 2008 Dec PubMed

A correlative light and electron microscopy method based on laser micropatterning and etching. Colombelli, J; Tängemo, C; Haselman, U; Antony, C; Stelzer, EH; Pepperkok, R; Reynaud, EG; Methods Mol Biol, 2008 PubMed

Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Keller, PJ; Schmidt, AD; Wittbrodt, J; Stelzer, EH; Science, 2008 Nov PubMed

The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis. Maier, P; Rathfelder, N; Maeder, CI; Colombelli, J; Stelzer, EH; Knop, M; EMBO J, 2008 Sep PubMed

In vivo imaging of the inflammatory receptor CD40 after cerebral ischemia using a fluorescent antibody. Klohs, J; Gräfe, M; Graf, K; Steinbrink, J; Dietrich, T; Stibenz, D; Bahmani, P; Kronenberg, G; Harms, C; Endres, M; Lindauer, U; Greger, K; Stelzer, EH; Dirnagl, U; Wunder, A; Stroke, 2008 Oct PubMed

Three-dimensional microtubule behavior in Xenopus egg extracts reveals four dynamic states and state-dependent elastic properties. Keller, PJ; Pampaloni, F; Lattanzi, G; Stelzer, EH; Biophys J, 2008 Aug PubMed

Resolution improvement by nonconfocal theta microscopy. Lindek, S; Stelzer, EH; Opt Lett, 1999 Nov PubMed

Optical levitation of absorbing particles with a nominally Gaussian laser beam. Huisken, J; Stelzer, EH; Opt Lett, 2002 Jul PubMed

Three-dimensional preparation and imaging reveal intrinsic microtubule properties. Keller, PJ; Pampaloni, F; Stelzer, EH; Nat Methods, 2007 Oct PubMed

The third dimension bridges the gap between cell culture and live tissue. Pampaloni, F; Reynaud, EG; Stelzer, EH; Nat Rev Mol Cell Biol, 2007 Oct PubMed

Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity. Kress, H; Stelzer, EH; Holzer, D; Buss, F; Griffiths, G; Rohrbach, A; Proc Natl Acad Sci U S A, 2007 Jul PubMed

Lateral modulation boosts image quality in single plane illumination fluorescence microscopy. Breuninger, T; Greger, K; Stelzer, EH; Opt Lett, 2007 Jul PubMed

Investigating relaxation processes in cells and developing organisms: from cell ablation to cytoskeleton nanosurgery. Colombelli, J; Reynaud, EG; Stelzer, EH; Methods Cell Biol, 2007 PubMed

Multi-view image fusion improves resolution in three-dimensional microscopy. Swoger, J; Verveer, P; Greger, K; Huisken, J; Stelzer, EH; Opt Express, 2007 Jun PubMed

Basic building units and properties of a fluorescence single plane illumination microscope. Greger, K; Swoger, J; Stelzer, EH; Rev Sci Instrum, 2007 Feb PubMed

Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM). Engelbrecht, CJ; Greger, K; Reynaud, EG; Krzic, U; Colombelli, J; Stelzer, EH; Opt Express, 2007 May PubMed

Three-dimensional optical manipulation using four collimated intersecting laser beams. Huisken, J; Swoger, J; Stelzer, EH; Opt Express, 2007 Apr PubMed

High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy. Verveer, PJ; Swoger, J; Pampaloni, F; Greger, K; Marcello, M; Stelzer, EH; Nat Methods, 2007 Apr PubMed

Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast. Taxis, C; Maeder, C; Reber, S; Rathfelder, N; Miura, K; Greger, K; Stelzer, EH; Knop, M; Traffic, 2006 Dec PubMed

Interferometric tracking of optically trapped probes behind structured surfaces: A phase correction method. Seitz, PC; Stelzer, EH; Rohrbach, A; Appl Opt, 2006 Oct PubMed

Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis. Gordon, O; Taxis, C; Keller, PJ; Benjak, A; Stelzer, EH; Simchen, G; Knop, M; EMBO J, 2006 Aug PubMed

[Laser nanosurgery in cell biology]. Colombelli, J; Pepperkok, R; Stelzer, EH; Reynaud, EG; Med Sci (Paris), PubMed

Resolution enhancement in a light-sheet-based microscope (SPIM). Engelbrecht, CJ; Stelzer, EH; Opt Lett, 2006 May PubMed

Life sciences require the third dimension. Keller, PJ; Pampaloni, F; Stelzer, EH; Curr Opin Cell Biol, 2006 Feb PubMed

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system. Taxis, C; Keller, P; Kavagiou, Z; Jensen, LJ; Colombelli, J; Bork, P; Stelzer, EH; Knop, M; J Cell Biol, 2005 Nov PubMed

In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Colombelli, J; Reynaud, EG; Rietdorf, J; Pepperkok, R; Stelzer, EH; Traffic, 2005 Dec PubMed

Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies. Kress, H; Stelzer, EH; Griffiths, G; Rohrbach, A; Phys Rev E Stat Nonlin Soft Matter Phys, 2005 Jun PubMed

Three-dimensional bead position histograms reveal single-molecule nanomechanics. Becker, NB; Altmann, SM; Scholz, T; Hörber, JK; Stelzer, EH; Rohrbach, A; Phys Rev E Stat Nonlin Soft Matter Phys, 2005 Feb PubMed

Stable chromosomal units determine the spatial and temporal organization of DNA replication. Sadoni, N; Cardoso, MC; Stelzer, EH; Leonhardt, H; Zink, D; J Cell Sci, 2004 Oct PubMed

Mechanical properties of single motor molecules studied by three-dimensional thermal force probing in optical tweezers. Jeney, S; Stelzer, EH; Grubmüller, H; Florin, EL; Chemphyschem, 2004 Aug PubMed

Optical sectioning deep inside live embryos by selective plane illumination microscopy. Huisken, J; Swoger, J; Del Bene, F; Wittbrodt, J; Stelzer, EH; Science, 2004 Aug PubMed

Multiple imaging axis microscopy improves resolution for thick-sample applications. Swoger, J; Huisken, J; Stelzer, EH; Opt Lett, 2003 Sep PubMed

Dynamic organization of the actin system in the motile cells of Dictyostelium. Bretschneider, T; Jonkman, J; Köhler, J; Medalia, O; Barisic, K; Weber, I; Stelzer, EH; Baumeister, W; Gerisch, G; J Muscle Res Cell Motil, 2002 PubMed

The distribution of active force generators controls mitotic spindle position. Grill, SW; Howard, J; Schäffer, E; Stelzer, EH; Hyman, AA; Science, 2003 Jul PubMed

Albumin-based drug delivery as novel therapeutic approach for rheumatoid arthritis. Wunder, A; Müller-Ladner, U; Stelzer, EH; Funk, J; Neumann, E; Stehle, G; Pap, T; Sinn, H; Gay, S; Fiehn, C; J Immunol, 2003 May PubMed

Three-dimensional tracking of small spheres in focused laser beams: influence of the detection angular aperture. Rohrbach, A; Kress, H; Stelzer, EH; Opt Lett, 2003 Mar PubMed

Resolution in optical microscopy. Jonkman, JE; Swoger, J; Kress, H; Rohrbach, A; Stelzer, EH; Methods Enzymol, 2003 PubMed

Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy. Swoger, J; Martínez-Corral, M; Huisken, J; Stelzer, EH; J Opt Soc Am A Opt Image Sci Vis, 2002 Sep PubMed

Beyond the diffraction limit? Stelzer, EH; Nature, 2002 Jun PubMed

Trapping forces, force constants, and potential depths for dielectric spheres in the presence of spherical aberrations. Rohrbach, A; Stelzer, EH; Appl Opt, 2002 May PubMed

Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. Rolls, MM; Hall, DH; Victor, M; Stelzer, EH; Rapoport, TA; Mol Biol Cell, 2002 May PubMed

Tailoring the axial shape of the point spread function using the Toraldo concept. Martinez-Corral, M; Caballero, M; Stelzer, EH; Swoger, J; Opt Express, 2002 Jan PubMed

Spatial partitioning of secretory cargo from Golgi resident proteins in live cells. White, J; Keller, P; Stelzer, EH; BMC Cell Biol, 2001 PubMed

Spindle positioning during the asymmetric first cell division of Caenorhabditis elegans embryos. Gönczy, P; Grill, S; Stelzer, EH; Kirkham, M; Hyman, AA; Novartis Found Symp, 2001 PubMed

Large-scale chromatin fibers of living cells display a discontinuous functional organization. Sadoni, N; Sullivan, KF; Weinzierl, P; Stelzer, EH; Zink, D; Chromosoma, 2001 Apr PubMed

Quantitative ER <--> Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cells. Dahm, T; White, J; Grill, S; Füllekrug, J; Stelzer, EH; Mol Biol Cell, 2001 May PubMed

Optical trapping of dielectric particles in arbitrary fields. Rohrbach, A; Stelzer, EH; J Opt Soc Am A Opt Image Sci Vis, 2001 Apr PubMed

Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo. Grill, SW; Gönczy, P; Stelzer, EH; Hyman, AA; Nature, 2001 Feb PubMed

Rab6 coordinates a novel Golgi to ER retrograde transport pathway in live cells. White, J; Johannes, L; Mallard, F; Girod, A; Grill, S; Reinsch, S; Keller, P; Tzschaschel, B; Echard, A; Goud, B; Stelzer, EH; J Cell Biol, 1999 Nov PubMed

The BioImage Database Project: organizing multidimensional biological images in an object-relational database. Carazo, JM; Stelzer, EH; J Struct Biol, PubMed

Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light. Pralle, A; Prummer, M; Florin, EL; Stelzer, EH; Hörber, JK; Microsc Res Tech, 1999 Mar PubMed

Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. Storrie, B; White, J; Röttger, S; Stelzer, EH; Suganuma, T; Nilsson, T; J Cell Biol, 1998 Dec PubMed

Organising multi-dimensional biological image information: the BioImage Database. Carazo, JM; Stelzer, EH; Engel, A; Fita, I; Henn, C; Machtynger, J; McNeil, P; Shotton, DM; Chagoyen, M; de Alarcón, PA; Fritsch, R; Heymann, JB; Kalko, S; Pittet, JJ; Rodriguez-Tomé, P; Boudier, T; Nucleic Acids Res, 1999 Jan PubMed

Structure and dynamics of human interphase chromosome territories in vivo. Zink, D; Cremer, T; Saffrich, R; Fischer, R; Trendelenburg, MF; Ansorge, W; Stelzer, EH; Hum Genet, 1998 Feb PubMed

Photonic force microscope based on optical tweezers and two-photon excitation for biological applications. Florin, EL; Pralle, A; Hörber, JK; Stelzer, EH; J Struct Biol, 1997 Jul PubMed

Confocal theta fluorescence microscopy with annular apertures. Lindek, S; Cremer, C; Stelzer, EH; Appl Opt, 1996 Jan PubMed

Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium-sapphire laser. Fischer, A; Cremer, C; Stelzer, EH; Appl Opt, 1995 Apr PubMed

Application of confocal laser microscopy and three-dimensional Voronoi diagrams for volume and surface estimates of interphase chromosomes. Eils, R; Bertin, E; Saracoglu, K; Rinke, B; Schröck, E; Parazza, F; Usson, Y; Robert-Nicoud, M; Stelzer, EH; Chassery, JM; J Microsc, 1995 Feb PubMed

The intracellular mobility of a viral membrane glycoprotein measured by confocal microscope fluorescence recovery after photobleaching. Storrie, B; Pepperkok, R; Stelzer, EH; Kreis, TE; J Cell Sci, 1994 May PubMed

Confocal microscopy with an increased detection aperture: type-B 4Pi confocal microscopy. Hell, SW; Stelzer, EH; Lindek, S; Cremer, C; Opt Lett, 1994 Feb PubMed

Apical and basal Forssman antigen in MDCK II cells: a morphological and quantitative study. Butor, C; Stelzer, EH; Sonnenberg, A; Davoust, J; Eur J Cell Biol, 1991 Dec PubMed

Confocal fluorescence microscopy in modern cell biology. Stelzer, EH; Wacker, I; De Mey, JR; Semin Cell Biol, 1991 Jun PubMed

The three-dimensional architecture of the mitotic spindle, analyzed by confocal fluorescence and electron microscopy. Merdes, A; Stelzer, EH; De Mey, J; J Electron Microsc Tech, 1991 May PubMed

Regulation of microtubule dynamics and nucleation during polarization in MDCK II cells. Bré, MH; Pepperkok, R; Hill, AM; Levilliers, N; Ansorge, W; Stelzer, EH; Karsenti, E; J Cell Biol, 1990 Dec PubMed

Unique geometry of actin-membrane anchorage sites in avian gizzard smooth muscle cells. Draeger, A; Stelzer, EH; Herzog, M; Small, JV; J Cell Sci, 1989 Dec PubMed

The subcellular organization of Madin-Darby canine kidney cells during the formation of a polarized epithelium. Bacallao, R; Antony, C; Dotti, C; Karsenti, E; Stelzer, EH; Simons, K; J Cell Biol, 1989 Dec PubMed

An antibody against secretogranin I (chromogranin B) is packaged into secretory granules. Rosa, P; Weiss, U; Pepperkok, R; Ansorge, W; Niehrs, C; Stelzer, EH; Huttner, WB; J Cell Biol, 1989 Jul PubMed

Preservation of biological specimens for observation in a confocal fluorescence microscope and operational principles of confocal fluorescence microscopy. Bacallao, R; Stelzer, EH; Methods Cell Biol, 1989 PubMed

Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells. van Meer, G; Stelzer, EH; Wijnaendts-van-Resandt, RW; Simons, K; J Cell Biol, 1987 Oct PubMed

This colleague is a member of the following groups:
  • Physical Biology