
Contact
Prof. Dr. Ernst Stelzer
Institute of Cell Biology and Neuroscience
Goethe University Frankfurt,
Riedberg Campus, BMLS
Max-von-Laue-Str. 15
60438 Frankfurt am Main
Germany
Tel +49 (0)69 798-42547
Fax +49 (0)69 798-42546
Administrative Assistant
Michaela Koller
Goethe University FrankfurtCampus Riedberg, BMLSMax-von-Laue-Str. 1560438 Frankfurt am MainGermany
Tel +49 (0)69 798-42545
Fax +49 (0)69 798-42546
Ernst Stelzer
Professor in Advanced Light Microscopy,Institute of Cell Biology and Neuroscience
Curriculum Vitae
| 1983 | Diploma in Physics, Max-Planck-Institute for Biophysics, Frankfurt am Main, Germany |
| 1987 | PhD, University of Heidelberg, Germany |
| 1987–1989 | Project leader EMBL Heidelberg, Germany |
| 1989–2009 | Group Leader, EMBL Heidelberg, Germany |
| since 2009 | Professor in Advanced Light Microscopy, Cluster of Excellence "Macromolecular Complexes", Goethe University Frankfurt |
Team
Ernst Stelzer, Group Leader
Elia Benito-Gutierrez, Postdoctoral Fellow
Fabian Härle, Software Engineer
Berit Langer, Technical Assistant
Hildegard Merkle, Administrative Assistant
Francesco Pampaloni, Staff Scientistmarko.reinhardt(at)physikalischebiologie.de
Patrick Theer, Physics Engineer
Daniel von Wangenheim, PhD Student
Nariman Ansari, Scientist and Project Leader ProMEBS
Publications
An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Rosquete, MR; von Wangenheim, D; Marhavý, P; Barbez, E; Stelzer, EH; Benková, E; Maizel, A; Kleine-Vehn, J; Curr Biol, 2013 May PubMed
Lateral root morphogenesis is dependent on the mechanical properties of the overlaying tissues. Lucas, M; Kenobi, K; von Wangenheim, D; Voβ, U; Swarup, K; De Smet, I; Van Damme, D; Lawrence, T; Péret, B; Moscardi, E; Barbeau, D; Godin, C; Salt, D; Guyomarc'h, S; Stelzer, EH; Maizel, A; Laplaze, L; Bennett, MJ; Proc Natl Acad Sci U S A, 2013 Mar PubMed
High-resolution deep imaging of live cellular spheroids with light-sheet-based fluorescence microscopy. Pampaloni, F; Ansari, N; Stelzer, EH; Cell Tissue Res, 2013 Apr PubMed
Quantitative 3D cell-based assay performed with cellular spheroids and fluorescence microscopy. Ansari, N; Müller, S; Stelzer, EH; Pampaloni, F; Methods Cell Biol, 2013 PubMed
Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope provides an improved signal to noise ratio. Greger, K; Neetz, MJ; Reynaud, EG; Stelzer, EH; Opt Express, 2011 Oct PubMed
Digital scanned laser light-sheet fluorescence microscopy (DSLM) of zebrafish and Drosophila embryonic development. Keller, PJ; Schmidt, AD; Wittbrodt, J; Stelzer, EH; Cold Spring Harb Protoc, 2011 Oct PubMed
Viscoelastic response of contractile filament bundles. Besser, A; Colombelli, J; Stelzer, EH; Schwarz, US; Phys Rev E Stat Nonlin Soft Matter Phys, 2011 May PubMed
High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy. Maizel, A; von Wangenheim, D; Federici, F; Haseloff, J; Stelzer, EH; Plant J, 2011 Oct PubMed
A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells. Tängemo, C; Ronchi, P; Colombelli, J; Haselmann, U; Simpson, JC; Antony, C; Stelzer, EH; Pepperkok, R; Reynaud, EG; J Cell Sci, 2011 Mar PubMed
ImFCS: a software for imaging FCS data analysis and visualization. Sankaran, J; Shi, X; Ho, LY; Stelzer, EH; Wohland, T; Opt Express, 2010 Dec PubMed
Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos. Redemann, S; Pecreaux, J; Goehring, NW; Khairy, K; Stelzer, EH; Hyman, AA; Howard, J; PLoS One, 2010 PubMed
Madin-Darby canine kidney cells are increased in aerobic glycolysis when cultured on flat and stiff collagen-coated surfaces rather than in physiological 3-D cultures. Pampaloni, F; Stelzer, EH; Leicht, S; Marcello, M; Proteomics, 2010 Oct PubMed
Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy. Keller, PJ; Schmidt, AD; Santella, A; Khairy, K; Bao, Z; Wittbrodt, J; Stelzer, EH; Nat Methods, 2010 Aug PubMed
Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Wohland, T; Shi, X; Sankaran, J; Stelzer, EH; Opt Express, 2010 May PubMed
Digital scanned laser light sheet fluorescence microscopy. Keller, PJ; Stelzer, EH; Cold Spring Harb Protoc, 2010 May PubMed
Nlcam modulates midline convergence during anterior neural plate morphogenesis. Brown, KE; Keller, PJ; Ramialison, M; Rembold, M; Stelzer, EH; Loosli, F; Wittbrodt, J; Dev Biol, 2010 Mar PubMed
A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation. Timinszky, G; Till, S; Hassa, PO; Hothorn, M; Kustatscher, G; Nijmeijer, B; Colombelli, J; Altmeyer, M; Stelzer, EH; Scheffzek, K; Hottiger, MO; Ladurner, AG; Nat Struct Mol Biol, 2009 Sep PubMed
Three-dimensional tissue models for drug discovery and toxicology. Pampaloni, F; Stelzer, EH; Masotti, A; Recent Pat Biotechnol, 2009 PubMed
Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage. Reynaud, EG; Krzic, U; Greger, K; Stelzer, EH; HFSP J, 2008 Oct PubMed
Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization. Colombelli, J; Besser, A; Kress, H; Reynaud, EG; Girard, P; Caussinus, E; Haselmann, U; Small, JV; Schwarz, US; Stelzer, EH; J Cell Sci, 2009 May PubMed
Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy. Keller, PJ; Stelzer, EH; Curr Opin Neurobiol, 2008 Dec PubMed
A correlative light and electron microscopy method based on laser micropatterning and etching. Colombelli, J; Tängemo, C; Haselman, U; Antony, C; Stelzer, EH; Pepperkok, R; Reynaud, EG; Methods Mol Biol, 2008 PubMed
Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Keller, PJ; Schmidt, AD; Wittbrodt, J; Stelzer, EH; Science, 2008 Nov PubMed
The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis. Maier, P; Rathfelder, N; Maeder, CI; Colombelli, J; Stelzer, EH; Knop, M; EMBO J, 2008 Sep PubMed
In vivo imaging of the inflammatory receptor CD40 after cerebral ischemia using a fluorescent antibody. Klohs, J; Gräfe, M; Graf, K; Steinbrink, J; Dietrich, T; Stibenz, D; Bahmani, P; Kronenberg, G; Harms, C; Endres, M; Lindauer, U; Greger, K; Stelzer, EH; Dirnagl, U; Wunder, A; Stroke, 2008 Oct PubMed
Three-dimensional microtubule behavior in Xenopus egg extracts reveals four dynamic states and state-dependent elastic properties. Keller, PJ; Pampaloni, F; Lattanzi, G; Stelzer, EH; Biophys J, 2008 Aug PubMed
Resolution improvement by nonconfocal theta microscopy. Lindek, S; Stelzer, EH; Opt Lett, 1999 Nov PubMed
Optical levitation of absorbing particles with a nominally Gaussian laser beam. Huisken, J; Stelzer, EH; Opt Lett, 2002 Jul PubMed
Three-dimensional preparation and imaging reveal intrinsic microtubule properties. Keller, PJ; Pampaloni, F; Stelzer, EH; Nat Methods, 2007 Oct PubMed
The third dimension bridges the gap between cell culture and live tissue. Pampaloni, F; Reynaud, EG; Stelzer, EH; Nat Rev Mol Cell Biol, 2007 Oct PubMed
Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity. Kress, H; Stelzer, EH; Holzer, D; Buss, F; Griffiths, G; Rohrbach, A; Proc Natl Acad Sci U S A, 2007 Jul PubMed
Lateral modulation boosts image quality in single plane illumination fluorescence microscopy. Breuninger, T; Greger, K; Stelzer, EH; Opt Lett, 2007 Jul PubMed
Investigating relaxation processes in cells and developing organisms: from cell ablation to cytoskeleton nanosurgery. Colombelli, J; Reynaud, EG; Stelzer, EH; Methods Cell Biol, 2007 PubMed
Multi-view image fusion improves resolution in three-dimensional microscopy. Swoger, J; Verveer, P; Greger, K; Huisken, J; Stelzer, EH; Opt Express, 2007 Jun PubMed
Basic building units and properties of a fluorescence single plane illumination microscope. Greger, K; Swoger, J; Stelzer, EH; Rev Sci Instrum, 2007 Feb PubMed
Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM). Engelbrecht, CJ; Greger, K; Reynaud, EG; Krzic, U; Colombelli, J; Stelzer, EH; Opt Express, 2007 May PubMed
Three-dimensional optical manipulation using four collimated intersecting laser beams. Huisken, J; Swoger, J; Stelzer, EH; Opt Express, 2007 Apr PubMed
High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy. Verveer, PJ; Swoger, J; Pampaloni, F; Greger, K; Marcello, M; Stelzer, EH; Nat Methods, 2007 Apr PubMed
Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast. Taxis, C; Maeder, C; Reber, S; Rathfelder, N; Miura, K; Greger, K; Stelzer, EH; Knop, M; Traffic, 2006 Dec PubMed
Interferometric tracking of optically trapped probes behind structured surfaces: A phase correction method. Seitz, PC; Stelzer, EH; Rohrbach, A; Appl Opt, 2006 Oct PubMed
Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis. Gordon, O; Taxis, C; Keller, PJ; Benjak, A; Stelzer, EH; Simchen, G; Knop, M; EMBO J, 2006 Aug PubMed
[Laser nanosurgery in cell biology]. Colombelli, J; Pepperkok, R; Stelzer, EH; Reynaud, EG; Med Sci (Paris), PubMed
Resolution enhancement in a light-sheet-based microscope (SPIM). Engelbrecht, CJ; Stelzer, EH; Opt Lett, 2006 May PubMed
Life sciences require the third dimension. Keller, PJ; Pampaloni, F; Stelzer, EH; Curr Opin Cell Biol, 2006 Feb PubMed
Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system. Taxis, C; Keller, P; Kavagiou, Z; Jensen, LJ; Colombelli, J; Bork, P; Stelzer, EH; Knop, M; J Cell Biol, 2005 Nov PubMed
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Colombelli, J; Reynaud, EG; Rietdorf, J; Pepperkok, R; Stelzer, EH; Traffic, 2005 Dec PubMed
Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies. Kress, H; Stelzer, EH; Griffiths, G; Rohrbach, A; Phys Rev E Stat Nonlin Soft Matter Phys, 2005 Jun PubMed
Three-dimensional bead position histograms reveal single-molecule nanomechanics. Becker, NB; Altmann, SM; Scholz, T; Hörber, JK; Stelzer, EH; Rohrbach, A; Phys Rev E Stat Nonlin Soft Matter Phys, 2005 Feb PubMed
Stable chromosomal units determine the spatial and temporal organization of DNA replication. Sadoni, N; Cardoso, MC; Stelzer, EH; Leonhardt, H; Zink, D; J Cell Sci, 2004 Oct PubMed
Mechanical properties of single motor molecules studied by three-dimensional thermal force probing in optical tweezers. Jeney, S; Stelzer, EH; Grubmüller, H; Florin, EL; Chemphyschem, 2004 Aug PubMed
Optical sectioning deep inside live embryos by selective plane illumination microscopy. Huisken, J; Swoger, J; Del Bene, F; Wittbrodt, J; Stelzer, EH; Science, 2004 Aug PubMed
Multiple imaging axis microscopy improves resolution for thick-sample applications. Swoger, J; Huisken, J; Stelzer, EH; Opt Lett, 2003 Sep PubMed
Dynamic organization of the actin system in the motile cells of Dictyostelium. Bretschneider, T; Jonkman, J; Köhler, J; Medalia, O; Barisic, K; Weber, I; Stelzer, EH; Baumeister, W; Gerisch, G; J Muscle Res Cell Motil, 2002 PubMed
The distribution of active force generators controls mitotic spindle position. Grill, SW; Howard, J; Schäffer, E; Stelzer, EH; Hyman, AA; Science, 2003 Jul PubMed
Albumin-based drug delivery as novel therapeutic approach for rheumatoid arthritis. Wunder, A; Müller-Ladner, U; Stelzer, EH; Funk, J; Neumann, E; Stehle, G; Pap, T; Sinn, H; Gay, S; Fiehn, C; J Immunol, 2003 May PubMed
Three-dimensional tracking of small spheres in focused laser beams: influence of the detection angular aperture. Rohrbach, A; Kress, H; Stelzer, EH; Opt Lett, 2003 Mar PubMed
Resolution in optical microscopy. Jonkman, JE; Swoger, J; Kress, H; Rohrbach, A; Stelzer, EH; Methods Enzymol, 2003 PubMed
Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy. Swoger, J; Martínez-Corral, M; Huisken, J; Stelzer, EH; J Opt Soc Am A Opt Image Sci Vis, 2002 Sep PubMed
Beyond the diffraction limit? Stelzer, EH; Nature, 2002 Jun PubMed
Trapping forces, force constants, and potential depths for dielectric spheres in the presence of spherical aberrations. Rohrbach, A; Stelzer, EH; Appl Opt, 2002 May PubMed
Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. Rolls, MM; Hall, DH; Victor, M; Stelzer, EH; Rapoport, TA; Mol Biol Cell, 2002 May PubMed
Tailoring the axial shape of the point spread function using the Toraldo concept. Martinez-Corral, M; Caballero, M; Stelzer, EH; Swoger, J; Opt Express, 2002 Jan PubMed
Spatial partitioning of secretory cargo from Golgi resident proteins in live cells. White, J; Keller, P; Stelzer, EH; BMC Cell Biol, 2001 PubMed
Spindle positioning during the asymmetric first cell division of Caenorhabditis elegans embryos. Gönczy, P; Grill, S; Stelzer, EH; Kirkham, M; Hyman, AA; Novartis Found Symp, 2001 PubMed
Large-scale chromatin fibers of living cells display a discontinuous functional organization. Sadoni, N; Sullivan, KF; Weinzierl, P; Stelzer, EH; Zink, D; Chromosoma, 2001 Apr PubMed
Quantitative ER <--> Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cells. Dahm, T; White, J; Grill, S; Füllekrug, J; Stelzer, EH; Mol Biol Cell, 2001 May PubMed
Optical trapping of dielectric particles in arbitrary fields. Rohrbach, A; Stelzer, EH; J Opt Soc Am A Opt Image Sci Vis, 2001 Apr PubMed
Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo. Grill, SW; Gönczy, P; Stelzer, EH; Hyman, AA; Nature, 2001 Feb PubMed
Rab6 coordinates a novel Golgi to ER retrograde transport pathway in live cells. White, J; Johannes, L; Mallard, F; Girod, A; Grill, S; Reinsch, S; Keller, P; Tzschaschel, B; Echard, A; Goud, B; Stelzer, EH; J Cell Biol, 1999 Nov PubMed
The BioImage Database Project: organizing multidimensional biological images in an object-relational database. Carazo, JM; Stelzer, EH; J Struct Biol, PubMed
Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light. Pralle, A; Prummer, M; Florin, EL; Stelzer, EH; Hörber, JK; Microsc Res Tech, 1999 Mar PubMed
Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. Storrie, B; White, J; Röttger, S; Stelzer, EH; Suganuma, T; Nilsson, T; J Cell Biol, 1998 Dec PubMed
Organising multi-dimensional biological image information: the BioImage Database. Carazo, JM; Stelzer, EH; Engel, A; Fita, I; Henn, C; Machtynger, J; McNeil, P; Shotton, DM; Chagoyen, M; de Alarcón, PA; Fritsch, R; Heymann, JB; Kalko, S; Pittet, JJ; Rodriguez-Tomé, P; Boudier, T; Nucleic Acids Res, 1999 Jan PubMed
Structure and dynamics of human interphase chromosome territories in vivo. Zink, D; Cremer, T; Saffrich, R; Fischer, R; Trendelenburg, MF; Ansorge, W; Stelzer, EH; Hum Genet, 1998 Feb PubMed
Photonic force microscope based on optical tweezers and two-photon excitation for biological applications. Florin, EL; Pralle, A; Hörber, JK; Stelzer, EH; J Struct Biol, 1997 Jul PubMed
Confocal theta fluorescence microscopy with annular apertures. Lindek, S; Cremer, C; Stelzer, EH; Appl Opt, 1996 Jan PubMed
Fluorescence of coumarins and xanthenes after two-photon absorption with a pulsed titanium-sapphire laser. Fischer, A; Cremer, C; Stelzer, EH; Appl Opt, 1995 Apr PubMed
Application of confocal laser microscopy and three-dimensional Voronoi diagrams for volume and surface estimates of interphase chromosomes. Eils, R; Bertin, E; Saracoglu, K; Rinke, B; Schröck, E; Parazza, F; Usson, Y; Robert-Nicoud, M; Stelzer, EH; Chassery, JM; J Microsc, 1995 Feb PubMed
The intracellular mobility of a viral membrane glycoprotein measured by confocal microscope fluorescence recovery after photobleaching. Storrie, B; Pepperkok, R; Stelzer, EH; Kreis, TE; J Cell Sci, 1994 May PubMed
Confocal microscopy with an increased detection aperture: type-B 4Pi confocal microscopy. Hell, SW; Stelzer, EH; Lindek, S; Cremer, C; Opt Lett, 1994 Feb PubMed
Apical and basal Forssman antigen in MDCK II cells: a morphological and quantitative study. Butor, C; Stelzer, EH; Sonnenberg, A; Davoust, J; Eur J Cell Biol, 1991 Dec PubMed
Confocal fluorescence microscopy in modern cell biology. Stelzer, EH; Wacker, I; De Mey, JR; Semin Cell Biol, 1991 Jun PubMed
The three-dimensional architecture of the mitotic spindle, analyzed by confocal fluorescence and electron microscopy. Merdes, A; Stelzer, EH; De Mey, J; J Electron Microsc Tech, 1991 May PubMed
Regulation of microtubule dynamics and nucleation during polarization in MDCK II cells. Bré, MH; Pepperkok, R; Hill, AM; Levilliers, N; Ansorge, W; Stelzer, EH; Karsenti, E; J Cell Biol, 1990 Dec PubMed
Unique geometry of actin-membrane anchorage sites in avian gizzard smooth muscle cells. Draeger, A; Stelzer, EH; Herzog, M; Small, JV; J Cell Sci, 1989 Dec PubMed
The subcellular organization of Madin-Darby canine kidney cells during the formation of a polarized epithelium. Bacallao, R; Antony, C; Dotti, C; Karsenti, E; Stelzer, EH; Simons, K; J Cell Biol, 1989 Dec PubMed
An antibody against secretogranin I (chromogranin B) is packaged into secretory granules. Rosa, P; Weiss, U; Pepperkok, R; Ansorge, W; Niehrs, C; Stelzer, EH; Huttner, WB; J Cell Biol, 1989 Jul PubMed
Preservation of biological specimens for observation in a confocal fluorescence microscope and operational principles of confocal fluorescence microscopy. Bacallao, R; Stelzer, EH; Methods Cell Biol, 1989 PubMed
Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells. van Meer, G; Stelzer, EH; Wijnaendts-van-Resandt, RW; Simons, K; J Cell Biol, 1987 Oct PubMed
- Physical Biology
