Research Groups

 

Publications of Volker Dötsch (Results: 151)

 

Structural and functional analysis of the GABARAP interaction motif (GIM).
EMBO reports
Rogov VV, Stolz A, Ravicahandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Löhr F, Wakatsuki S, Dötsch V, Dikic I, Dobson RC, McEwan DG PubMed
Jun 2017 Volume: Issue: Pages:

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.
Biophysical journal
Henrich E, Sörmann J, Eberhardt P, Peetz O, Mezhyrova J, Morgner N, Fendler K, Dötsch V, Wachtveitl J, Bernhard F, Bamann C PubMed
Apr 2017 Volume: Issue: Pages:

Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins.
Scientific reports
Rogov VV, Suzuki H, Marinković M, Lang V, Kato R, Kawasaki M, Buljubašić M, Šprung M, Rogova N, Wakatsuki S, Hamacher-Brady A, Dötsch V, Dikic I, Brady NR, Novak I PubMed
Apr 2017 Volume: 7 Issue: 1 Pages: 1131

Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2.
EMBO reports
Imre G, Berthelet J, Heering J, Kehrloesser S, Melzer IM, Lee BI, Thiede B, Dötsch V, Rajalingam K PubMed
May 2017 Volume: 18 Issue: 5 Pages: 733-744

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology.
eLife
Henrich E, Peetz O, Hein C, Laguerre A, Hoffmann B, Hoffmann J, Dötsch V, Bernhard F, Morgner N PubMed
Jan 2017 Volume: 6 Issue: Pages:

Acceleration of protein backbone NMR assignment by combinatorial labeling: Application to a small molecule binding study.
Biopolymers
Hein C, Löhr F, Schwarz D, Dötsch V PubMed
May 2017 Volume: 107 Issue: 5 Pages:

Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins.
The EMBO journal
Stolz A, Putyrski M, Kutle I, Huber J, Wang C, Major V, Sidhu SS, Youle RJ, Rogov VV, Dötsch V, Ernst A, Dikic I PubMed
Feb 2017 Volume: 36 Issue: 4 Pages: 549-564

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn(2).
Journal of neurochemistry
Waberer L, Henrich E, Peetz O, Morgner N, Dötsch V, Bernhard F, Volknandt W PubMed
Jan 2017 Volume: 140 Issue: 2 Pages: 280-293

Mechanism of TAp73 inhibition by ΔNp63 and structural basis of p63/p73 hetero-tetramerization.
Cell death and differentiation
Gebel J, Luh LM, Coutandin D, Osterburg C, Löhr F, Schäfer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Güntert P, Knapp S, Dötsch V PubMed
Dec 2016 Volume: 23 Issue: 12 Pages: 1930-1940

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins.
Structure (London, England : 1993)
Laguerre A, Löhr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM, Bernhard F, Dötsch V PubMed
Oct 2016 Volume: 24 Issue: 10 Pages: 1830-1841

Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family.
Cell death and differentiation
Kehrloesser S, Osterburg C, Tuppi M, Schäfer B, Vousden KH, Dötsch V PubMed
Dec 2016 Volume: 23 Issue: 12 Pages: 1952-1960

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression.
Biochemistry
Focke PJ, Hein C, Hoffmann B, Matulef K, Bernhard F, Dötsch V, Valiyaveetil FI PubMed
Aug 2016 Volume: 55 Issue: 30 Pages: 4212-9

The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.
Molecular cell
von Delbrück M, Kniss A, Rogov VV, Pluska L, Bagola K, Löhr F, Güntert P, Sommer T, Dötsch V PubMed
Jun 2016 Volume: 62 Issue: 6 Pages: 918-28

Structural Evolution and Dynamics of the p53 Proteins.
Cold Spring Harbor perspectives in medicine
Chillemi G, Kehrloesser S, Bernassola F, Desideri A, Dötsch V, Levine AJ, Melino G PubMed
Apr 2017 Volume: 7 Issue: 4 Pages:

Quality control in oocytes by p63 is based on a spring-loaded activation mechanism on the molecular and cellular level.
eLife
Coutandin D, Osterburg C, Srivastav RK, Sumyk M, Kehrloesser S, Gebel J, Tuppi M, Hannewald J, Schäfer B, Salah E, Mathea S, Müller-Kuller U, Doutch J, Grez M, Knapp S, Dötsch V PubMed
Mar 2016 Volume: 5 Issue: Pages:

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments.
Biochimica et biophysica acta
Rues RB, Dötsch V, Bernhard F PubMed
Jun 2016 Volume: 1858 Issue: 6 Pages: 1306-16

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway.
The Journal of biological chemistry
Henrich E, Ma Y, Engels I, Münch D, Otten C, Schneider T, Henrichfreise B, Sahl HG, Dötsch V, Bernhard F PubMed
Jan 2016 Volume: 291 Issue: 5 Pages: 2535-46

Labeling of membrane proteins by cell-free expression.
Methods in enzymology
LaGuerre A, Löhr F, Bernhard F, Dötsch V PubMed
2015 Volume: 565 Issue: Pages: 367-88

Structural investigations of the p53/p73 homologs from the tunicate species Ciona intestinalis reveal the sequence requirements for the formation of a tetramerization domain.
Protein science : a publication of the Protein Society
Heering J, Jonker HR, Löhr F, Schwalbe H, Dötsch V PubMed
Feb 2016 Volume: 25 Issue: 2 Pages: 410-22

Probing metallo-β-lactamases with molecular fragments identified by consensus docking.
Bioorganic & medicinal chemistry letters
Klingler FM, Moser D, Büttner D, Wichelhaus TA, Löhr F, Dötsch V, Proschak E PubMed
Nov 2015 Volume: 25 Issue: 22 Pages: 5243-6

TECPR2 Cooperates with LC3C to Regulate COPII-Dependent ER Export.
Molecular cell
Stadel D, Millarte V, Tillmann KD, Huber J, Tamin-Yecheskel BC, Akutsu M, Demishtein A, Ben-Zeev B, Anikster Y, Perez F, Dötsch V, Elazar Z, Rogov V, Farhan H, Behrends C PubMed
Oct 2015 Volume: 60 Issue: 1 Pages: 89-104

Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing.
The Journal of biological chemistry
Mehler M, Eckert CE, Busche A, Kulhei J, Michaelis J, Becker-Baldus J, Wachtveitl J, Dötsch V, Glaubitz C PubMed
Nov 2015 Volume: 290 Issue: 46 Pages: 27712-22

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing.
Biological chemistry
Merk H, Rues RB, Gless C, Beyer K, Dong F, Dötsch V, Gerrits M, Bernhard F PubMed
Sep 2015 Volume: 396 Issue: 9-10 Pages: 1097-107

Structure and Biophysical Characterization of the S-Adenosylmethionine-dependent O-Methyltransferase PaMTH1, a Putative Enzyme Accumulating during Senescence of Podospora anserina.
The Journal of biological chemistry
Chatterjee D, Kudlinzki D, Linhard V, Saxena K, Schieborr U, Gande SL, Wurm JP, Wöhnert J, Abele R, Rogov VV, Dötsch V, Osiewacz HD, Sreeramulu S, Schwalbe H PubMed
Jun 2015 Volume: 290 Issue: 26 Pages: 16415-30

An extended combinatorial 15N, 13Cα, and 13C' labeling approach to protein backbone resonance assignment.
Journal of biomolecular NMR
Löhr F, Tumulka F, Bock C, Abele R, Dötsch V PubMed
Jul 2015 Volume: 62 Issue: 3 Pages: 263-79

Membrane protein production in Escherichia coli cell-free lysates.
FEBS letters
Henrich E, Hein C, Dötsch V, Bernhard F PubMed
Jul 2015 Volume: 589 Issue: 15 Pages: 1713-22

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs.
Methods in enzymology
Henrich E, Dötsch V, Bernhard F PubMed
2015 Volume: 556 Issue: Pages: 351-69

FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors.
International journal of cancer
Schwamb B, Pick R, Fernández SB, Völp K, Heering J, Dötsch V, Bösser S, Jung J, Beinoraviciute-Kellner R, Wesely J, Zörnig I, Hammerschmidt M, Nowak M, Penzel R, Zatloukal K, Joos S, Rieker RJ, Agaimy A, Söder S, Reid-Lombardo KM, Kendrick ML, Bardsley MR, Hayashi Y, Asuzu DT, Syed SA, Ordog T, Zörnig M PubMed
Sep 2015 Volume: 137 Issue: 6 Pages: 1318-29

CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.
Molecular cell
Genau HM, Huber J, Baschieri F, Akutsu M, Dötsch V, Farhan H, Rogov V, Behrends C PubMed
Mar 2015 Volume: 57 Issue: 6 Pages: 995-1010

Cell-free expression of G-protein-coupled receptors.
Methods in molecular biology (Clifton, N.J.)
Orbán E, Proverbio D, Haberstock S, Dötsch V, Bernhard F PubMed
2015 Volume: 1261 Issue: Pages: 171-95

Co-translational stabilization of insoluble proteins in cell-free expression systems.
Methods in molecular biology (Clifton, N.J.)
Kai L, Orbán E, Henrich E, Proverbio D, Dötsch V, Bernhard F PubMed
2015 Volume: 1258 Issue: Pages: 125-43

Time-shared experiments for efficient assignment of triple-selectively labeled proteins.
Journal of magnetic resonance (San Diego, Calif. : 1997)
Löhr F, Laguerre A, Bock C, Reckel S, Connolly PJ, Abdul-Manan N, Tumulka F, Abele R, Moore JM, Dötsch V PubMed
Nov 2014 Volume: 248 Issue: Pages: 81-95

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets.
Biological chemistry
Rues RB, Orbán E, Dötsch V, Bernhard F PubMed
Dec 2014 Volume: 395 Issue: 12 Pages: 1425-34

Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.
Biochimica et biophysica acta
Schröder M, Häfner AK, Hofmann B, Rådmark O, Tumulka F, Abele R, Dötsch V, Steinhilber D PubMed
Oct 2014 Volume: 1842 Issue: 10 Pages: 1538-47

In-cell NMR and EPR spectroscopy of biomacromolecules.
Angewandte Chemie (International ed. in English)
Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V PubMed
Sep 2014 Volume: 53 Issue: 39 Pages: 10300-14

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.
Cellular and molecular life sciences : CMLS
Boland C, Li D, Shah STA, Haberstock S, Dötsch V, Bernhard F, Caffrey M PubMed
Dec 2014 Volume: 71 Issue: 24 Pages: 4895-4910

Crystal structure of a PCP/Sfp complex reveals the structural basis for carrier protein posttranslational modification.
Chemistry & biology
Tufar P, Rahighi S, Kraas FI, Kirchner DK, Löhr F, Henrich E, Köpke J, Dikic I, Güntert P, Marahiel MA, Dötsch V PubMed
Apr 2014 Volume: 21 Issue: 4 Pages: 552-562

Peak picking NMR spectral data using non-negative matrix factorization.
BMC bioinformatics
Tikole S, Jaravine V, Rogov V, Dötsch V, Güntert P PubMed
Feb 2014 Volume: 15 Issue: Pages: 46

Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy.
Molecular cell
Rogov V, Dötsch V, Johansen T, Kirkin V PubMed
Jan 2014 Volume: 53 Issue: 2 Pages: 167-78

High-level cell-free production of membrane proteins with nanodiscs.
Methods in molecular biology (Clifton, N.J.)
Roos C, Kai L, Haberstock S, Proverbio D, Ghoshdastider U, Ma Y, Filipek S, Wang X, Dötsch V, Bernhard F PubMed
2014 Volume: 1118 Issue: Pages: 109-30

SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions.
PloS one
Zettler J, Eppmann S, Busche A, Dikovskaya D, Dötsch V, Mootz HD, Sonntag T PubMed
2013 Volume: 8 Issue: 9 Pages: e72925

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.
Journal of biomolecular NMR
Tumulka F, Roos C, Löhr F, Bock C, Bernhard F, Dötsch V, Abele R PubMed
Oct 2013 Volume: 57 Issue: 2 Pages: 141-54

Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition.
Journal of the American Chemical Society
Luh LM, Hänsel R, Löhr F, Kirchner DK, Krauskopf K, Pitzius S, Schäfer B, Tufar P, Corbeski I, Güntert P, Dötsch V PubMed
Sep 2013 Volume: 135 Issue: 37 Pages: 13796-803

How to switch a master switch.
eLife
Hein C, Wittinghofer A, Dötsch V PubMed
Jul 2013 Volume: 2 Issue: Pages: e01159

Endoplasmic reticulum targeting and insertion of tail-anchored membrane proteins by the GET pathway.
Cold Spring Harbor perspectives in biology
Denic V, Dötsch V, Sinning I PubMed
Aug 2013 Volume: 5 Issue: 8 Pages: a013334

Structural basis for phosphorylation-triggered autophagic clearance of Salmonella.
The Biochemical journal
Rogov VV, Suzuki H, Fiskin E, Wild P, Kniss A, Rozenknop A, Kato R, Kawasaki M, McEwan DG, Löhr F, Güntert P, Dikic I, Wakatsuki S, Dötsch V PubMed
Sep 2013 Volume: 454 Issue: 3 Pages: 459-66

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments.
Biochimica et biophysica acta
Proverbio D, Roos C, Beyermann M, Orbán E, Dötsch V, Bernhard F PubMed
Sep 2013 Volume: 1828 Issue: 9 Pages: 2182-92

Artificial environments for the co-translational stabilization of cell-free expressed proteins.
PloS one
Kai L, Dötsch V, Kaldenhoff R, Bernhard F PubMed
2013 Volume: 8 Issue: 2 Pages: e56637

High-resolution insight into G-overhang architecture.
Journal of the American Chemical Society
Hänsel R, Löhr F, Trantirek L, Dötsch V PubMed
Feb 2013 Volume: 135 Issue: 7 Pages: 2816-24

Modified lipid and protein dynamics in nanodiscs.
Biochimica et biophysica acta
Mörs K, Roos C, Scholz F, Wachtveitl J, Dötsch V, Bernhard F, Glaubitz C PubMed
Apr 2013 Volume: 1828 Issue: 4 Pages: 1222-9

Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies.
Journal of biomolecular NMR
Stehle J, Scholz F, Löhr F, Reckel S, Roos C, Blum M, Braun M, Glaubitz C, Dötsch V, Wachtveitl J, Schwalbe H PubMed
Dec 2012 Volume: 54 Issue: 4 Pages: 401-13

A method for integrative structure determination of protein-protein complexes.
Bioinformatics (Oxford, England)
Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velázquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dötsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A PubMed
Dec 2012 Volume: 28 Issue: 24 Pages: 3282-9

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening.
Protein expression and purification
Ma Y, Ghoshdastider U, Wang J, Ye W, Dötsch V, Filipek S, Bernhard F, Wang X PubMed
Dec 2012 Volume: 86 Issue: 2 Pages: 120-6

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.
Biochimica et biophysica acta
Roos C, Zocher M, Müller D, Münch D, Schneider T, Sahl HG, Scholz F, Wachtveitl J, Ma Y, Proverbio D, Henrich E, Dötsch V, Bernhard F PubMed
Dec 2012 Volume: 1818 Issue: 12 Pages: 3098-106

Loss of p63 and its microRNA-205 target results in enhanced cell migration and metastasis in prostate cancer.
Proceedings of the National Academy of Sciences of the United States of America
Tucci P, Agostini M, Grespi F, Markert EK, Terrinoni A, Vousden KH, Muller PA, Dötsch V, Kehrloesser S, Sayan BS, Giaccone G, Lowe SW, Takahashi N, Vandenabeele P, Knight RA, Levine AJ, Melino G PubMed
Sep 2012 Volume: 109 Issue: 38 Pages: 15312-7

Investigation of quadruplex structure under physiological conditions using in-cell NMR.
Topics in current chemistry
Hänsel R, Foldynová-Trantírková S, Dötsch V, Trantírek L PubMed
2013 Volume: 330 Issue: Pages: 47-65

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.
Molecular membrane biology
Roos C, Kai L, Proverbio D, Ghoshdastider U, Filipek S, Dötsch V, Bernhard F PubMed
Feb 2013 Volume: 30 Issue: 1 Pages: 75-89

Requirements on paramagnetic relaxation enhancement data for membrane protein structure determination by NMR.
Structure (London, England : 1993)
Gottstein D, Reckel S, Dötsch V, Güntert P PubMed
Jun 2012 Volume: 20 Issue: 6 Pages: 1019-27

Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis.
The EMBO journal
Imre G, Heering J, Takeda AN, Husmann M, Thiede B, zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dötsch V, Rajalingam K PubMed
May 2012 Volume: 31 Issue: 11 Pages: 2615-28

Fast automated NMR spectroscopy of short-lived biological samples.
Chembiochem : a European journal of chemical biology
Tikole S, Jaravine V, Rogov VV, Rozenknop A, Schmöe K, Löhr F, Dötsch V, Güntert P PubMed
May 2012 Volume: 13 Issue: 7 Pages: 964-7

A universal expression tag for structural and functional studies of proteins.
Chembiochem : a European journal of chemical biology
Rogov VV, Rozenknop A, Rogova NY, Löhr F, Tikole S, Jaravine V, Güntert P, Dikic I, Dötsch V PubMed
May 2012 Volume: 13 Issue: 7 Pages: 959-63

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.
Protein expression and purification
Haberstock S, Roos C, Hoevels Y, Dötsch V, Schnapp G, Pautsch A, Bernhard F PubMed
Apr 2012 Volume: 82 Issue: 2 Pages: 308-16

In-cell solid-state NMR as a tool to study proteins in large complexes.
Chembiochem : a European journal of chemical biology
Reckel S, Lopez JJ, Löhr F, Glaubitz C, Dötsch V PubMed
Mar 2012 Volume: 13 Issue: 4 Pages: 534-7

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment.
Journal of biomolecular NMR
Löhr F, Reckel S, Karbyshev M, Connolly PJ, Abdul-Manan N, Bernhard F, Moore JM, Dötsch V PubMed
Mar 2012 Volume: 52 Issue: 3 Pages: 197-210

Single-molecule force spectroscopy from nanodiscs: an assay to quantify folding, stability, and interactions of native membrane proteins.
ACS nano
Zocher M, Roos C, Wegmann S, Bosshart PD, Dötsch V, Bernhard F, Müller DJ PubMed
Jan 2012 Volume: 6 Issue: 1 Pages: 961-71

Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase.
ACS chemical biology
Busche A, Gottstein D, Hein C, Ripin N, Pader I, Tufar P, Eisman EB, Gu L, Walsh CT, Sherman DH, Löhr F, Güntert P, Dötsch V PubMed
Feb 2012 Volume: 7 Issue: 2 Pages: 378-86

Solution NMR structure of proteorhodopsin.
Angewandte Chemie (International ed. in English)
Reckel S, Gottstein D, Stehle J, Löhr F, Verhoefen MK, Takeda M, Silvers R, Kainosho M, Glaubitz C, Wachtveitl J, Bernhard F, Schwalbe H, Güntert P, Dötsch V PubMed
Dec 2011 Volume: 50 Issue: 50 Pages: 11942-6

Systems for the cell-free synthesis of proteins.
Methods in molecular biology (Clifton, N.J.)
Kai L, Roos C, Haberstock S, Proverbio D, Ma Y, Junge F, Karbyshev M, Dötsch V, Bernhard F PubMed
2012 Volume: 800 Issue: Pages: 201-25

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.
The Journal of biological chemistry
Ma Y, Münch D, Schneider T, Sahl HG, Bouhss A, Ghoshdastider U, Wang J, Dötsch V, Wang X, Bernhard F PubMed
Nov 2011 Volume: 286 Issue: 45 Pages: 38844-53

Cell-free expression and assembly of ATP synthase.
Journal of molecular biology
Matthies D, Haberstock S, Joos F, Dötsch V, Vonck J, Bernhard F, Meier T PubMed
Oct 2011 Volume: 413 Issue: 3 Pages: 593-603

The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization.
The Journal of biological chemistry
Keller T, Egenberger B, Gorboulev V, Bernhard F, Uzelac Z, Gorbunov D, Wirth C, Koppatz S, Dötsch V, Hunte C, Sitte HH, Koepsell H PubMed
Oct 2011 Volume: 286 Issue: 43 Pages: 37874-86

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification.
The Journal of biological chemistry
Rath P, Demange P, Saurel O, Tropis M, Daffé M, Dötsch V, Ghazi A, Bernhard F, Milon A PubMed
Sep 2011 Volume: 286 Issue: 37 Pages: 32525-32

Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.
Science (New York, N.Y.)
Stefer S, Reitz S, Wang F, Wild K, Pang YY, Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F, Denic V, Dötsch V, Sinning I PubMed
Aug 2011 Volume: 333 Issue: 6043 Pages: 758-62

Differential altered stability and transcriptional activity of ΔNp63 mutants in distinct ectodermal dysplasias.
Journal of cell science
Browne G, Cipollone R, Lena AM, Serra V, Zhou H, van Bokhoven H, Dötsch V, Merico D, Mantovani R, Terrinoni A, Knight RA, Candi E, Melino G PubMed
Jul 2011 Volume: 124 Issue: Pt 13 Pages: 2200-7

Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy.
Journal of biomolecular NMR
Löhr F, Reckel S, Stefer S, Dötsch V, Schmidt JM PubMed
Jun 2011 Volume: 50 Issue: 2 Pages: 167-90

Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1.
Journal of molecular biology
Rozenknop A, Rogov VV, Rogova NY, Löhr F, Güntert P, Dikic I, Dötsch V PubMed
Jul 2011 Volume: 410 Issue: 3 Pages: 477-87

Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth.
Science (New York, N.Y.)
Wild P, Farhan H, McEwan DG, Wagner S, Rogov VV, Brady NR, Richter B, Korac J, Waidmann O, Choudhary C, Dötsch V, Bumann D, Dikic I PubMed
Jul 2011 Volume: 333 Issue: 6039 Pages: 228-33

Mutation in SAM domain of TP63 is associated with nonsyndromic cleft lip and palate and cleft palate.
American journal of medical genetics. Part A
Kantaputra PN, Malaivijitnond S, Vieira AR, Heering J, Dötsch V, Khankasikum T, Sripathomsawat W PubMed
Jun 2011 Volume: 155A Issue: 6 Pages: 1432-6

How to create a specific recognition for an unspecific interaction.
Structure (London, England : 1993)
Dötsch V PubMed
May 2011 Volume: 19 Issue: 5 Pages: 601-2

Quality control in oocytes: domain-domain interactions regulate the activity of p63.
Cell cycle (Georgetown, Tex.)
Deutsch GB, Zielonka EM, Coutandin D, Dötsch V PubMed
Jun 2011 Volume: 10 Issue: 12 Pages: 1884-5

Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy.
Angewandte Chemie (International ed. in English)
Krstić I, Hänsel R, Romainczyk O, Engels JW, Dötsch V, Prisner TF PubMed
May 2011 Volume: 50 Issue: 22 Pages: 5070-4

Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements.
Journal of structural biology
Kodama Y, Reese ML, Shimba N, Ono K, Kanamori E, Dötsch V, Noguchi S, Fukunishi Y, Suzuki E, Shimada I, Takahashi H PubMed
Jun 2011 Volume: 174 Issue: 3 Pages: 434-42

Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB.
Structure (London, England : 1993)
Schmöe K, Rogov VV, Rogova NY, Löhr F, Güntert P, Bernhard F, Dötsch V PubMed
Apr 2011 Volume: 19 Issue: 4 Pages: 577-87

A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF.
The Journal of biological chemistry
Rogov VV, Rogova NY, Bernhard F, Löhr F, Dötsch V PubMed
May 2011 Volume: 286 Issue: 21 Pages: 18775-83

The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions.
Nucleic acids research
Hänsel R, Löhr F, Foldynová-Trantírková S, Bamberg E, Trantírek L, Dötsch V PubMed
Jul 2011 Volume: 39 Issue: 13 Pages: 5768-75

DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramer.
Cell
Deutsch GB, Zielonka EM, Coutandin D, Weber TA, Schäfer B, Hannewald J, Luh LM, Durst FG, Ibrahim M, Hoffmann J, Niesen FH, Sentürk A, Kunkel H, Brutschy B, Schleiff E, Knapp S, Acker-Palmer A, Grez M, McKeon F, Dötsch V PubMed
Feb 2011 Volume: 144 Issue: 4 Pages: 566-76

Phenotypic analysis of Arg227 mutations of TP63 with emphasis on dental phenotype and micturition difficulties in EEC syndrome.
American journal of medical genetics. Part A
Sripathomsawat W, Tanpaiboon P, Heering J, Dötsch V, Hennekam RC, Kantaputra P PubMed
Jan 2011 Volume: 155A Issue: 1 Pages: 228-32

Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.
Journal of biomolecular NMR
Hefke F, Bagaria A, Reckel S, Ullrich SJ, Dötsch V, Glaubitz C, Güntert P PubMed
Feb 2011 Volume: 49 Issue: 2 Pages: 75-84

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes.
PloS one
Kai L, Kaldenhoff R, Lian J, Zhu X, Dötsch V, Bernhard F, Cen P, Xu Z PubMed
Sep 2010 Volume: 5 Issue: 9 Pages: e12972

NmerA of Tn501 mercuric ion reductase: structural modulation of the pKa values of the metal binding cysteine thiols.
Biochemistry
Ledwidge R, Hong B, Dötsch V, Miller SM PubMed
Oct 2010 Volume: 49 Issue: 41 Pages: 8988-98

Tracing the protectors path from the germ line to the genome.
Proceedings of the National Academy of Sciences of the United States of America
Coutandin D, Ou HD, Löhr F, Dötsch V PubMed
Aug 2010 Volume: 107 Issue: 35 Pages: 15318-25

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.
New biotechnology
Junge F, Haberstock S, Roos C, Stefer S, Proverbio D, Dötsch V, Bernhard F PubMed
Apr 2011 Volume: 28 Issue: 3 Pages: 262-71

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor.
Journal of structural biology
Junge F, Luh LM, Proverbio D, Schäfer B, Abele R, Beyermann M, Dötsch V, Bernhard F PubMed
Oct 2010 Volume: 172 Issue: 1 Pages: 94-106

Structural investigation of the C-terminal catalytic fragment of presenilin 1.
Proceedings of the National Academy of Sciences of the United States of America
Sobhanifar S, Schneider B, Löhr F, Gottstein D, Ikeya T, Mlynarczyk K, Pulawski W, Ghoshdastider U, Kolinski M, Filipek S, Güntert P, Bernhard F, Dötsch V PubMed
May 2010 Volume: 107 Issue: 21 Pages: 9644-9

Strategies for the cell-free expression of membrane proteins.
Methods in molecular biology (Clifton, N.J.)
Reckel S, Sobhanifar S, Durst F, Löhr F, Shirokov VA, Dötsch V, Bernhard F PubMed
2010 Volume: 607 Issue: Pages: 187-212

Cell-free expression profiling of E. coli inner membrane proteins.
Proteomics
Schwarz D, Daley D, Beckhaus T, Dötsch V, Bernhard F PubMed
May 2010 Volume: 10 Issue: 9 Pages: 1762-79

Membrane protein expression in cell-free systems.
Methods in molecular biology (Clifton, N.J.)
Schneider B, Junge F, Shirokov VA, Durst F, Schwarz D, Dötsch V, Bernhard F PubMed
2010 Volume: 601 Issue: Pages: 165-86

Nix is a selective autophagy receptor for mitochondrial clearance.
EMBO reports
Novak I, Kirkin V, McEwan DG, Zhang J, Wild P, Rozenknop A, Rogov V, Löhr F, Popovic D, Occhipinti A, Reichert AS, Terzic J, Dötsch V, Ney PA, Dikic I PubMed
Jan 2010 Volume: 11 Issue: 1 Pages: 45-51

Ubiquitin linkages make a difference.
Nature structural & molecular biology
Dikic I, Dötsch V PubMed
Dec 2009 Volume: 16 Issue: 12 Pages: 1209-10

The molecular pharmacology and in vivo activity of 2-(4-chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase.
The Journal of pharmacology and experimental therapeutics
Koeberle A, Rossi A, Zettl H, Pergola C, Dehm F, Bauer J, Greiner C, Reckel S, Hoernig C, Northoff H, Bernhard F, Dötsch V, Sautebin L, Schubert-Zsilavecz M, Werz O PubMed
Mar 2010 Volume: 332 Issue: 3 Pages: 840-8

NMR studies reveal the role of biomembranes in modulating ligand binding and release by intracellular bile acid binding proteins.
Journal of molecular biology
Pedò M, Löhr F, D'Onofrio M, Assfalg M, Dötsch V, Molinari H PubMed
Dec 2009 Volume: 394 Issue: 5 Pages: 852-63

Evaluation of parameters critical for observing nucleic acids inside living Xenopus laevis oocytes by in-cell NMR spectroscopy.
Journal of the American Chemical Society
Hänsel R, Foldynová-Trantírková S, Löhr F, Buck J, Bongartz E, Bamberg E, Schwalbe H, Dötsch V, Trantírek L PubMed
Nov 2009 Volume: 131 Issue: 43 Pages: 15761-8

On track to tenure-track.
EMBO reports
Dötsch V PubMed
Sep 2009 Volume: 10 Issue: 9 Pages: 936-7

Cell-free expression and stable isotope labelling strategies for membrane proteins.
Journal of biomolecular NMR
Sobhanifar S, Reckel S, Junge F, Schwarz D, Kai L, Karbyshev M, Löhr F, Bernhard F, Dötsch V PubMed
Jan 2010 Volume: 46 Issue: 1 Pages: 33-43

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.
Angewandte Chemie (International ed. in English)
Busche AE, Aranko AS, Talebzadeh-Farooji M, Bernhard F, Dötsch V, Iwaï H PubMed
2009 Volume: 48 Issue: 33 Pages: 6128-31

A cost-effective amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae.
Journal of biomolecular structure & dynamics
Foldynová-Trantirková S, Matulová J, Dötsch V, Löhr F, Cirstea I, Alexandov K, Breitling R, Lukes J, Trantírek L PubMed
Jun 2009 Volume: 26 Issue: 6 Pages: 755-61

Modulation of the Rcs-mediated signal transfer by conformational flexibility.
Biochemical Society transactions
Rogov VV, Schmöe K, Löhr F, Rogova NY, Bernhard F, Dötsch V PubMed
Dec 2008 Volume: 36 Issue: Pt 6 Pages: 1427-32

The better tag remains unseen.
Journal of the American Chemical Society
Durst FG, Ou HD, Löhr F, Dötsch V, Straub WE PubMed
Nov 2008 Volume: 130 Issue: 45 Pages: 14932-3

Production of membrane proteins using cell-free expression systems.
Proteomics
Schwarz D, Dötsch V, Bernhard F PubMed
Oct 2008 Volume: 8 Issue: 19 Pages: 3933-46

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase.
Nature
Koglin A, Löhr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Güntert P, Wagner G, Walsh CT, Marahiel MA, Dötsch V PubMed
Aug 2008 Volume: 454 Issue: 7206 Pages: 907-11

Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins.
Proceedings of the National Academy of Sciences of the United States of America
Reckel S, Sobhanifar S, Schneider B, Junge F, Schwarz D, Durst F, Löhr F, Güntert P, Bernhard F, Dötsch V PubMed
Jun 2008 Volume: 105 Issue: 24 Pages: 8262-7

Cell free expression and functional reconstitution of eukaryotic drug transporters.
Biochemistry
Keller T, Schwarz D, Bernhard F, Dötsch V, Hunte C, Gorboulev V, Koepsell H PubMed
Apr 2008 Volume: 47 Issue: 15 Pages: 4552-64

Investigation of proteins in living bacteria with in-cell NMR experiments.
Topics in current chemistry
Dötsch V PubMed
2008 Volume: 273 Issue: Pages: 203-14

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.
Nature protocols
Schwarz D, Junge F, Durst F, Frölich N, Schneider B, Reckel S, Sobhanifar S, Dötsch V, Bernhard F PubMed
2007 Volume: 2 Issue: 11 Pages: 2945-57

Reprint of "Cell-free production of G protein-coupled receptors for functional and structural studies" [J. Struct. Biol. 158 (2007) 482-493].
Journal of structural biology
Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, Haase W, Hahn S, Dötsch V, Bernhard F PubMed
Aug 2007 Volume: 159 Issue: 2 Pages: 194-205

Cell-free production of integral membrane proteins on a preparative scale.
Methods in molecular biology (Clifton, N.J.)
Klammt C, Schwarz D, Dötsch V, Bernhard F PubMed
2007 Volume: 375 Issue: Pages: 57-78

Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes.
The EMBO journal
Ikeda F, Hecker CM, Rozenknop A, Nordmeier RD, Rogov V, Hofmann K, Akira S, Dötsch V, Dikic I PubMed
Jul 2007 Volume: 26 Issue: 14 Pages: 3451-62

E3-independent monoubiquitination of ubiquitin-binding proteins.
Molecular cell
Hoeller D, Hecker CM, Wagner S, Rogov V, Dötsch V, Dikic I PubMed
Jun 2007 Volume: 26 Issue: 6 Pages: 891-8

Structural evolution of C-terminal domains in the p53 family.
The EMBO journal
Ou HD, Löhr F, Vogel V, Mäntele W, Dötsch V PubMed
Jul 2007 Volume: 26 Issue: 14 Pages: 3463-73

Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy.
Nature protocols
Serber Z, Selenko P, Hänsel R, Reckel S, Löhr F, Ferrell JE, Wagner G, Dötsch V PubMed
2006 Volume: 1 Issue: 6 Pages: 2701-9

Cell-free production of G protein-coupled receptors for functional and structural studies.
Journal of structural biology
Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, Haase W, Hahn S, Dötsch V, Bernhard F PubMed
Jun 2007 Volume: 158 Issue: 3 Pages: 482-93

Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteins.
Journal of biomolecular NMR
Löhr F, Hänsel R, Rogov VV, Dötsch V PubMed
Mar 2007 Volume: 37 Issue: 3 Pages: 205-24

The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a.
Nature structural & molecular biology
Reese ML, Dakoji S, Bredt DS, Dötsch V PubMed
Feb 2007 Volume: 14 Issue: 2 Pages: 155-63

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.
Journal of biomolecular NMR
Rogov VV, Löhr F, Rogova N, Klammt C, Koglin A, Bernhard F, Dötsch V PubMed
Jun 2007 Volume: 38 Issue: 2 Pages: 165

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.
Journal of molecular biology
Rogov VV, Rogova NY, Bernhard F, Koglin A, Löhr F, Dötsch V PubMed
Nov 2006 Volume: 364 Issue: 1 Pages: 68-79

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies.
Methods (San Diego, Calif.)
Schwarz D, Klammt C, Koglin A, Löhr F, Schneider B, Dötsch V, Bernhard F PubMed
Apr 2007 Volume: 41 Issue: 4 Pages: 355-69

Cell-free expression as an emerging technique for the large scale production of integral membrane protein.
The FEBS journal
Klammt C, Schwarz D, Löhr F, Schneider B, Dötsch V, Bernhard F PubMed
Sep 2006 Volume: 273 Issue: 18 Pages: 4141-53

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.
Magnetic resonance in chemistry : MRC
Koglin A, Klammt C, Trbovic N, Schwarz D, Schneider B, Schäfer B, Löhr F, Bernhard F, Dötsch V PubMed
Jul 2006 Volume: 44 Spec No Issue: Pages: S17-23

NMR assignment of the L27 heterodimer from LIN-2 and LIN-7 scaffold proteins.
Journal of biomolecular NMR
Petrosky KY, Löhr F, Dötsch V PubMed
2006 Volume: 36 Suppl 1 Issue: Pages: 15

Conformational switches modulate protein interactions in peptide antibiotic synthetases.
Science (New York, N.Y.)
Koglin A, Mofid MR, Löhr F, Schäfer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dötsch V PubMed
Apr 2006 Volume: 312 Issue: 5771 Pages: 273-6

One functional switch mediates reversible and irreversible inactivation of a herpesvirus protease.
Biochemistry
Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS PubMed
Mar 2006 Volume: 45 Issue: 11 Pages: 3572-9

Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex.
The Journal of biological chemistry
Kelly AE, Kranitz H, Dötsch V, Mullins RD PubMed
Apr 2006 Volume: 281 Issue: 15 Pages: 10589-97

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system.
The FEBS journal
Klammt C, Schwarz D, Fendler K, Haase W, Dötsch V, Bernhard F PubMed
Dec 2005 Volume: 272 Issue: 23 Pages: 6024-38

Induced structure of a helical switch as a mechanism to regulate enzymatic activity.
Nature structural & molecular biology
Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS PubMed
Nov 2005 Volume: 12 Issue: 11 Pages: 1019-20

Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues.
Journal of biomolecular NMR
Löhr F, Rogov VV, Shi M, Bernhard F, Dötsch V PubMed
Aug 2005 Volume: 32 Issue: 4 Pages: 309-28

A methylation-dependent electrostatic switch controls DNA repair and transcriptional activation by E. coli ada.
Molecular cell
He C, Hus JC, Sun LJ, Zhou P, Norman DP, Dötsch V, Wei H, Gross JD, Lane WS, Wagner G, Verdine GL PubMed
Oct 2005 Volume: 20 Issue: 1 Pages: 117-29

Efficient strategy for the rapid backbone assignment of membrane proteins.
Journal of the American Chemical Society
Trbovic N, Klammt C, Koglin A, Löhr F, Bernhard F, Dötsch V PubMed
Oct 2005 Volume: 127 Issue: 39 Pages: 13504-5

A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes.
The Journal of biological chemistry
Petrosky KY, Ou HD, Löhr F, Dötsch V, Lim WA PubMed
Nov 2005 Volume: 280 Issue: 46 Pages: 38528-36

In-cell NMR spectroscopy.
Chembiochem : a European journal of chemical biology
Reckel S, Löhr F, Dötsch V PubMed
Sep 2005 Volume: 6 Issue: 9 Pages: 1601-6

In-cell NMR spectroscopy.
Methods in enzymology
Serber Z, Corsini L, Durst F, Dötsch V PubMed
2005 Volume: 394 Issue: Pages: 17-41

Optimization of 13C direct detection NMR methods.
Journal of biomolecular NMR
Shimba N, Kovacs H, Stern AS, Nomura AM, Shimada I, Hoch JC, Craik CS, Dötsch V PubMed
Oct 2004 Volume: 30 Issue: 2 Pages: 175-9

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.
Journal of molecular biology
Rogov VV, Bernhard F, Löhr F, Dötsch V PubMed
Oct 2004 Volume: 343 Issue: 4 Pages: 1035-48

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain.
Journal of biomolecular NMR
V Rogov V, Löhr F, Bernhard F, Dötsch V PubMed
Sep 2004 Volume: 30 Issue: 1 Pages: 103-4

Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
Journal of the American Chemical Society
Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dötsch V PubMed
Jun 2004 Volume: 126 Issue: 22 Pages: 7119-25

High level cell-free expression and specific labeling of integral membrane proteins.
European journal of biochemistry
Klammt C, Löhr F, Schäfer B, Haase W, Dötsch V, Rüterjans H, Glaubitz C, Bernhard F PubMed
Feb 2004 Volume: 271 Issue: 3 Pages: 568-80

Fast mapping of protein-protein interfaces by NMR spectroscopy.
Journal of the American Chemical Society
Reese ML, Dötsch V PubMed
Nov 2003 Volume: 125 Issue: 47 Pages: 14250-1

Quantitative identification of the protonation state of histidines in vitro and in vivo.
Biochemistry
Shimba N, Serber Z, Ledwidge R, Miller SM, Craik CS, Dötsch V PubMed
Aug 2003 Volume: 42 Issue: 30 Pages: 9227-34

Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction.
Journal of the American Chemical Society
Shimba N, Stern AS, Craik CS, Hoch JC, Dötsch V PubMed
Mar 2003 Volume: 125 Issue: 9 Pages: 2382-3

A C-terminal inhibitory domain controls the activity of p63 by an intramolecular mechanism.
Molecular and cellular biology
Serber Z, Lai HC, Yang A, Ou HD, Sigal MS, Kelly AE, Darimont BD, Duijf PH, Van Bokhoven H, McKeon F, Dötsch V PubMed
Dec 2002 Volume: 22 Issue: 24 Pages: 8601-11

Low-conductivity buffers for high-sensitivity NMR measurements.
Journal of the American Chemical Society
Kelly AE, Ou HD, Withers R, Dötsch V PubMed
Oct 2002 Volume: 124 Issue: 40 Pages: 12013-9

Gain-of-function mutation in ADULT syndrome reveals the presence of a second transactivation domain in p63.
Human molecular genetics
Duijf PH, Vanmolkot KR, Propping P, Friedl W, Krieger E, McKeon F, Dötsch V, Brunner HG, van Bokhoven H PubMed
Apr 2002 Volume: 11 Issue: 7 Pages: 799-804