A comprehensive review on light sheet fluorescence microscopy
12 November 2021. Light sheet microscopes illuminate only a thin slice of a fluorescent sample and observe the emission from this plane perpendicularly. This arrangement reduces phototoxicity to such an extent that completely new experiments become possible. Light sheet fluorescence microscopy is one of the cornerstones of the BMLS-hosted Frankfurt Centre for Advanced Light Microscopy. In their recent Nature Reviews Methods Primer, which is directed at newcomers and experts alike, Ernst Stelzer and Frederic Strobl discuss the history, technical background, applications and future trends of this technology together with international colleagues. Read the Primer here.
Contacts
Frederic Strobl and Ernst Stelzer, Buchmann Institute for Molecular Life Sciences and Institute of Cell Biology and Neuroscience, Goethe University Frankfurt, Frankfurt/Main, frederic.strobl@physikalischebiologie.de, ernst.stelzer@physikalischebiologie.de